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. 2020 Sep 29;21(19):7181. doi: 10.3390/ijms21197181

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell viability, executioner 3/7 caspase activity, and UPR induction in SH-Syn_T. (A) Live cell count by Trypan blue staining of SH-Syn_T and SH-Mock_T. Data are presented as the means ± SD from three independent experiments, each performed in triplicate. Data were analyzed by a two-tailed Student’s t-test (* p < 0.05). (B) Analysis of caspase 3/7 activity in SH-Syn_T and SH-Mock_T. Data are presented as the means ± SD from two independent experiments, each performed in triplicate. Data were analyzed by a two-tailed Student’s t-test (* p < 0.05). (C) BiP, ATF4, CHOP, and r-XBP1 mRNA quantification by qPCR in SH-Syn_T and SH-Mock_T. Data are presented as the mean ± SD of three independent experiments, each performed in duplicate. Data were analyzed by a Mann–Whitney Rank Sum Test (* p < 0.05, ** p < 0.001).