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. 2020 Sep 29;21(19):7181. doi: 10.3390/ijms21197181

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Interaction of CLU and αSyn in SH-Syn. (A) Detection of CLU in the SH-Syn intracellular protein fraction (Lysate) immunoprecipitated with the anti-CLU antibody. (B) Detection of αSyn in the SH-Syn intracellular protein fraction (Lysate) immunoprecipitated with the anti-CLU antibody. Immunoprecipitation with IgG was performed as a negative control. IP: Immunoprecipitated fraction; S: Fraction containing the proteins that do not interact with CLU; Lysate: 1% Triton X-100 protein soluble fraction of SH-Syn; IgG-HC: IgG Heavy chain; IgG-LC: IgG Light chain.