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. 2020 Sep 29;21(19):7181. doi: 10.3390/ijms21197181

Figure 8.

Figure 8

Effects of CLU down-regulation in SH-Syn. (A) Detection of CLU in the 1% Triton X-100 soluble fraction of SH-SynsiRNA and SH-SynNC. Blots are representative of experiments repeated three times. β-actin was used as the loading control. (B) Detection of αSyn in the 1% Triton X-100 soluble fraction, in the pellet fraction, and in the culture medium of SH-SynsiRNA and SH-SynNC. Blots are representative of experiments repeated two times. β-actin was used as the loading control; an asterisk represents non-specific bands. (C) Detection of Hsp27, Hsp70, and Hsp90 in the 1% Triton X-100 soluble fraction and in the pellet fraction of SH-SynsiRNA and SH-SynNC. Blots are representative of experiments repeated three times. β-actin was used as the loading control. (D) The cell viability of SH-SynsiRNA and SH-SynNC by the WST-1 assay. Data are shown as the mean ± SD of two independent experiments, each performed in triplicate. The difference between groups is not statistically significant. (E) Analysis of the caspase 3/7 activity in SH-SynsiRNA and SH-SynNC. Data are presented as the mean ± SD of two independent experiments, each performed in triplicate. The difference between groups is not statistically significant. (F) BiP, ATF4, CHOP, and r-XBP1 mRNA quantification by qPCR in SH-SynsiRNA and SH-SynNC. Data are presented as the mean ± SD of three independent experiments, each performed in duplicate. The difference between groups is not statistically significant.