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. 2020 Sep 24;21(19):7036. doi: 10.3390/ijms21197036

Figure 10.

Figure 10

MC4R, MRAP2b, AgRP, AgRP2, POMCa1, POMCa2, and POMCb expression in tilapia hypothalamus and pituitary tissues. (A) The expression levels of MC4R, MRAP2b, AgRP, AgRP2, POMCa1, POMCa2, and POMCb mRNA were estimated using the RNA-Seq data from the hypothalamus of growing tilapia (30-day-old, n = 5). RNA-seq data were calculated as TPM (transcript per million). (B) The expression levels of MRAP2b, AgRP, AgRP2, POMCa1, POMCa2, and POMCb in the pituitary were estimated using the RNA-Seq data from the pituitary of 30-day-old tilapia (n = 2). (C) Quantitative real-time RT-PCR assay of AgRP expression in the hypothalamus of 30-day-old tilapia under 3-day (Fast day3) or 6-day fasting (Fast day6) compared with that of control (Con day3, Con day6) (i.e., tilapia with free access to food). White pillars and black pillars indicated the control group and fasting group, respectively. Each data point represents the mean ± SEM of four tilapia (n = 4). ***, p < 0.001 vs. control (Con).