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UGT74E2 catalyzes indole-3-butyric acid (IBA) glycosylation in rice. (A) RT-PCR analysis of the mRNA levels of UGT74E2 in transgenic rice plants. (B) HPLC profiling of IBA glucose conjugates (IBA-Glc) extracted from seven-day-old wild type (WT) and UGT74E2-overexpressed lines after 0 μM IBA or 100 μM IBA treatment for 12 h. (C) Relative IBA-Glc contents in UGT74E2 transgenic rice plants (* p < 0.05). (D) LC-MS confirmation of IBA-glucose ester under positive ion mode.
