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. 2020 Sep 30;21(19):7239. doi: 10.3390/ijms21197239

Figure 4.

Figure 4

UGT74E2 altered IBA and ABA contents in germinating rice seeds. (A) The contents of IBA in germinating WT and UGT74E2 transgenic rice seeds after being imbibed for 24 h. (B) The contents of ABA in germinating WT and UGT74E2 transgenic rice seeds after being imbibed for 24 h. (C) Expression of OsABI3 and OsABI5 in germinating seeds in WT and UGT74E2 transgenic rice. Gene expression was normalized to the reference gene OsActin. For each experiment, three replicates were done; data are presented as means ± SD. Student’s t-test for each genotype was performed (* p < 0.05, ** p < 0.01). (D) Role of ABI3 and ABI5 genes in the ABA signaling pathway during seed germination.