Figure 7.

Accumulation of ROS under NaCl and PEG treatments. (A) Two-week-old seedlings were subjected to nitroblue tetrazoliun (NBT) and diaminobenzidine (DAB) staining after treatment with NaCl and polyethylene glycol (PEG) for 24 h. (scale bars = 1 cm) (B) Genes encoding antioxidant enzymes are downregulated upon UGT74E2 overexpression under abiotic stress. For RNA extraction, seven-day-old rice plants were subjected to 200 mM NaCl and 15% PEG treatment for 12 h. Gene expression was normalized to the reference gene OsActin. For each experiment, three replicates were done; data are presented as means ± SD. Student’s t-test for each genotype was performed (** p < 0.01).