Table 3.
List of primer sequences and their reaction conditions used in the present study.
| Sequence (5′ to 3′) | Probe | Tm (°C) | Size (bp) | ||
|---|---|---|---|---|---|
| Real-time RT-PCRa | |||||
| GAPDH | Forward | AGCCACATCGCTCAGACAC | #60 | 60 | 66 |
| Reverse | GCCCAATACGACCAAATCC | ||||
| SMAD3 | Forward | GTCTGCAAGATCCCACCA | #79 | 59 | 88 |
| Reverse | AGCCCTGGTTGACCGACT | ||||
| Quantitative Methylation-Specific PCR | |||||
| ACTB | Forward | TGGTGATGGAGGAGGTTTAGTAAGT | 60 | 132 | |
| Reverse | AACCAATAAAACCTACTCCTCCCTTAA | ||||
| TagMan probe | ACCACCACCCAACACACAATAACAAACACA | ||||
| SMAD3 | Forward | GAATAAGGTCGTTAGTTATTATCGT | 54.48 | 172 | |
| Reverse | AATCAAATCTACCCGAATCGAA | ||||
| TaqMan probe | GAAAGAAAGAAAGAAAGTAAATTTTATTTTTAAGCG | ||||