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. 2020 Sep 30;21(19):7225. doi: 10.3390/ijms21197225

Figure 1.

Figure 1

Effects of high-fat diet and leptin deficiency on weight gain, fat weight, and adiposity. (A) Body weight of mice after 20 weeks of feeding (n = 10 per group). (B) Fat mass weight measured by Echo Magnetic Resonance Imaging (EchoMRI). (C,D) Representative photographs (C) and weight (D) of epididymal adipose tissue. Scale bar = 1 cm. (E) Representative images (200×) of hematoxylin and eosin (H&E) staining in adipose tissue sections. Scale bar = 100 μm. (F) Adipocyte size (n = 3). (G) Number of crown-like structures (CLSs) measured by H&E-stained epididymal fat (n = 3). (H,I) Quantitative RT-PCR analysis of tumor necrosis factor-α (Tnf-α), monocyte chemoattractant protein 1 (Mcp1), CC chemokine ligand 5 (CCL5), C-C motif receptor 5 (CCR5), interleukin-10 (Il-10), and arginase 1 (Arg1) mRNA. Data are presented as the means ± standard of error of the mean (SEM). * p < 0.05 versus control (CTL) mice; † p < 0.05 versus diet-induced obesity (DIO) mice. CTL (black circle), DIO (blue square), ob/ob (red triangle).