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. 2020 Sep 30;21(19):7225. doi: 10.3390/ijms21197225

Figure 3.

Figure 3

Effects of high-fat diet and leptin deficiency on inflammation and apoptosis in adipose tissue. (A) Western blot analysis of SHIP1 protein expression. (B) Representative immunostained SHIP1 and Cluster of Differentiation 68 (CD68) in serial adipose tissue sections (200×). (C) Representative immunofluorescent images (200×) of SHIP1 (green) and perilipin 1 (red). (D) Representative immunofluorescent images (200×) of SHIP1 (red) and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) (green). (E) Western blot analysis of Bax and Bcl-2 protein. Graph indicates the Bax/Bcl-2 ratio. (F,G) Western blot analysis of phosphoinositide 3-kinase (PI3K),total protein kinase B (t-Akt), and phosphorylated Akt (p-Akt) protein expression, using α-tubulin as a reference protein. Data are presented as the means ± SEM. * p < 0.05 versus CTL mice; † p < 0.05 versus DIO mice. Scale bar = 50 μm. CTL (black circle), DIO (blue square), ob/ob (red triangle).