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. 2020 Sep 24;25(19):4392. doi: 10.3390/molecules25194392

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of TTPBgp12 from Yersinia phage φYeO3-12 using 12% SDS-PAGE. The lanes are as follows: (1) prestained protein ladder—mid-range molecular weight (10–180 kDa) (Nippon Genetics); (2) crude extract; (3) the flow through fraction and (4) pooled fractions eluted with 250 mM imidazole after the first round of nickel-immobilized affinity column; (5) TTPBgp12 fractions after Tobacco Etch Virus (TEV) protease cleavage; (6) pooled fractions eluted with 250 mM imidazole after the second round of Ni²⁺-affinity chromatography; (7) denaturated and undenaturated (8) TTPBgp12 fractions after ammonium sulfate precipitation; (9) denaturated and undenaturated (10) TTPBgp12 solution after gel-filtration chromatography.