Table 1.
Sample | Tm (°C) | |||
---|---|---|---|---|
Fluorescence Ratio 350 nm/330 nm |
First Derivative | Scattering | ||
#1 | #2 | |||
TTPBgp12/melibiose | 36.20 | 47.45 | 32.51 | 39.43 |
TTPBgp12/galactose | 35.30 | 46.40 | 32.02 | 36.93 |
TTPBgp12/glucose | 37.21 | 46.98 | NA | 33.76 |
TTPBgp12/maltose | 37.64 | NA | 30.85 | 42.11 |
TTPBgp12/β-lactose | 35.13 | 46.96 | 32.46 | 39.19 |
TTPBgp12/α-lactose | 34.74 | 47.42 | 30.99 | 40.31 |
TTPBgp12/GalNAc | 43.65 | 75.19 | 38.73 | 45.65 |
TTPBgp12/GlcNAc | 41.66 | NA | 28.23 | 56.61 |
TTPBgp12 | 36.04 | 46.46 | 33.13 | 37.89 |
TFPgp17/melibiose | 81.16 | NA | 72.83 | 64.64 |
TFPgp17/galactose | 80.30 | NA | 72.88 | 63.78 |
TFPgp17/glucose | 80.86 | NA | 72.89 | 65.71 |
TFPgp17/maltose | 77.49 | NA | 69.03 | 46.99 |
TFPgp17/β-lactose | 81.45 | NA | 72.57 | 64.19 |
TFPgp17/α-lactose | 81.51 | NA | 73.01 | 64.66 |
TFPgp17/GalNAc | 80.41 | NA | 71.96 | 80.45 |
TFPgp17/GlcNAc | 78.29 | NA | 69.28 | 56.06 |
TFPgp17 | 80.70 | NA | 72.15 | 65.55 |
Each sample contained 0.25 mg/mL of the tail protein and 0.15 mg/mL of the sugar.