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. 2020 Sep 24;25(19):4392. doi: 10.3390/molecules25194392

Table 1.

TTPBgp12 and TFPgp17 thermal unfolding and aggregation transitions midpoints (Tm (°C)) obtained from a melting scan of both proteins mixed with sugars, showing the fluorescence ratio 350 nm/330 nm), the first derivative onset and scattering.

Sample Tm (°C)
Fluorescence Ratio
350 nm/330 nm
First Derivative Scattering
#1 #2
TTPBgp12/melibiose 36.20 47.45 32.51 39.43
TTPBgp12/galactose 35.30 46.40 32.02 36.93
TTPBgp12/glucose 37.21 46.98 NA 33.76
TTPBgp12/maltose 37.64 NA 30.85 42.11
TTPBgp12/β-lactose 35.13 46.96 32.46 39.19
TTPBgp12/α-lactose 34.74 47.42 30.99 40.31
TTPBgp12/GalNAc 43.65 75.19 38.73 45.65
TTPBgp12/GlcNAc 41.66 NA 28.23 56.61
TTPBgp12 36.04 46.46 33.13 37.89
TFPgp17/melibiose 81.16 NA 72.83 64.64
TFPgp17/galactose 80.30 NA 72.88 63.78
TFPgp17/glucose 80.86 NA 72.89 65.71
TFPgp17/maltose 77.49 NA 69.03 46.99
TFPgp17/β-lactose 81.45 NA 72.57 64.19
TFPgp17/α-lactose 81.51 NA 73.01 64.66
TFPgp17/GalNAc 80.41 NA 71.96 80.45
TFPgp17/GlcNAc 78.29 NA 69.28 56.06
TFPgp17 80.70 NA 72.15 65.55

Each sample contained 0.25 mg/mL of the tail protein and 0.15 mg/mL of the sugar.