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. 2020 Sep 25;21(19):7056. doi: 10.3390/ijms21197056

Figure 3.

Figure 3

Comparison of cGMP signals at the outer mitochondrial membrane (OMM) of NVM from control and mdx mice. (A) Top: schematic representation of the OMM-targeted cGMP FRET reporter OMM-cGi500. T.70 indicates translocase outer mitochondria membrane protein TOM 70. Bottom: at the left, representative images of NVM from control and mdx mice expressing OMM-cGi500. Co-staining with the mitochondria specific dye mitotracker is shown in the central panel and the overlay is shown at the right. (B) Mean relative values for CFP to YFP fluorescence intensity ratio recorded in unstimulated NVM from control and mdx mice expressing OMM-cGi500. n = 4. (C) Mean FRET change recorded in NVM expressing OMM-cGi500 on application of family-selective cGMP-PDE inhibitors as indicated n = 4. (D) Mean FRET change recorded in NVM from control or mdx mice expressing OMM-cGi500 on application of the NO donor SNAP in combination with the cGC stimulator Bay-41. n = 4. (E) Mean FRET change recorded in NVM expressing OMM-cGi500 on application of family-selective cGMP-PDE inhibitors, as indicated, in cells pre-treated as in (D) n = 4. For all data, mean ± SEM are shown. *** = p < 0.001. Size bar: 10 µm.