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. 2020 Sep 30;21(19):7224. doi: 10.3390/ijms21197224

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of FAS1 deletions on conidiogenesis, appressorial formation and vegetative growth. (A) Light microscopy of conidia cultured on oatmeal agar 10 days post-incubation at 26 °C. Basal appendage formation observed where conidia is attached to conidiophores. Magnification 100× light microscope. Bar = 10 µm. (B) Appressorium formation of conidia inoculated onto plastic cover slips in a moist chamber at 24 °C. (C) Size measurement of conidia length and width in micrometer, 10 days post-inoculation at temperature of 26 °C. Error bars represent the standard deviation. (D) Appearance of wildtype, mutant fas1-1 and mutant fas1-2 spore formation after 10 days of incubation on complete medium at temperature of 26 °C. Magnification 100x light microscope. (E) Scanning electron microscope image of wildtype, mutant fas1-1 and mutant fas1-2 spore after 10 days of incubation on complete medium at temperature of 26 °C. Magnification 1000× and 2000× of scanning electron microscope.

Effect of FAS1 deletions on conidiogenesis, appressorial formation and vegetative growth. (A) Light microscopy of conidia cultured on oatmeal agar 10 days post-incubation at 26 °C. Basal appendage formation observed where conidia is attached to conidiophores. Magnification 100× light microscope. Bar = 10 µm. (B) Appressorium formation of conidia inoculated onto plastic cover slips in a moist chamber at 24 °C. (C) Size measurement of conidia length and width in micrometer, 10 days post-inoculation at temperature of 26 °C. Error bars represent the standard deviation. (D) Appearance of wildtype, mutant fas1-1 and mutant fas1-2 spore formation after 10 days of incubation on complete medium at temperature of 26 °C. Magnification 100x light microscope. (E) Scanning electron microscope image of wildtype, mutant fas1-1 and mutant fas1-2 spore after 10 days of incubation on complete medium at temperature of 26 °C. Magnification 1000× and 2000× of scanning electron microscope.