Figure 9.

Gene expression of PEX6 and ICL1 expression in the wildtype strain S6, Δfas1-1 and Δfas1-2 mutants grown in complete media for 48 h and then transferred to a new flask of complete medium, or minimal medium containing 50 mM olive oil for 24 h. Expression profiles of PEX6 (peroxisomal biogenesis) and ICL1 (glyoxylate cycle) genes were analyzed. Gene expression data of PEX6 and ICL1, obtained from quantitative RT-PCR analysis, were normalized by using with housekeeping gene EF1α as an internal control, calculated using three independent biological experiments with three technical replicates for each independent experiment, as reported above. Double asterisks represent significant difference (** p ≤ 0.01).