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. 2020 Oct 22;10:18014. doi: 10.1038/s41598-020-74917-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Consistency of nuclei proportion derived from staining-based nuclei counts with mRNA-based estimates, without and with adjustment for neural connectivity. (A) The neuron/non-neuron ratios between the nuclei-staining-based estimate and the unadjusted mRNA-based estimate. The 18 points on each tier of the y axis are the results from using the 3 RNA-Seq datasets on six Allen Human Brain Atlas (AHBA) donors. The three levels of nuclei ratios are for each region (cerebrum, cerebellum and brainstem). (B) The data are the same as in A except that the mRNA neuron/non-neuron ratios were divided by the projection volume, which adjusts for the differences in neuron length among different regions.