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. 2020 Oct 6;21(19):7367. doi: 10.3390/ijms21197367

Figure 2.

Figure 2

Cytoplasmic membrane permeabilization of E. coli ML35p by cePrAMPs. Peptide-mediated membrane permeabilization at ½ × MIC, 1 × MIC and 2 × MIC. Inner membrane permeabilization was monitored as an increase in absorbance at 405 nm by O-nitrophenol, a product of the hydrolysis of the impermeable, chromogenic substrate ONPG after 30′ and 60′ incubation. The membranolytic peptide antibiotic colistin has been used for comparison. The complete kinetics of permeabilization are shown in Figure S1.