Table 1.
Physicochemical properties of TFPs and experimental conditions for NMR measurements.
| Protein | pI | Charges (+/−/His) a | HI b | Labeling | Conc., mM | pH | T, °C | Freq., MHz c | PDB | Ref |
|---|---|---|---|---|---|---|---|---|---|---|
| SLURP-1 | 5.2 | 8/10/1 | 0.04 | 13C, 15N | 0.3 | 4.7 | 37 | 800 | 6ZZE 6ZZF | This work |
| Lypd6 | 5.3 | 11/15/3 | −0.58 | 13C, 15N/15N d | 0.1 | 7.0 | 30/37 d | 600/800 | 6IB6 | This work |
| Lypd6b | 6.7 | 10/11/8 | −0.68 | 13C, 15N | 0.2 | 5.5 | 30/37 d | 600/800 | 6ZSO | This work |
| Lynx2 | 4.5 | 5/8/0 | 0.07 | 13C, 15N | 0.25/0.07 d | 6.7 | 45/37 d | 600/800 | 6ZSS | This work |
| Lynx1 | 8.1 | 8/6/2 | −0.32 | 15N | 0.5 | 5.3 | 25 | 800 | 2L03 | [11] |
| SLURP-2 | 6.5 | 5/6/5 | 0.10 | 13C, 15N | 0.5 e/0.08 d | 4.8 | 37 | 600 | 2N99 | [32] |
| WTX-P33A | 8.9 | 13/7/1 | −0.39 | 15N | 0.5 | 3.0 | 30 | 700 | 2MJ0 | [43] |
| NTII | 8.7 | 10/6/2 | −1.10 | 15N | 1.0 | 5.0 | 30 | 800 | 2MJ4 | [44] |
a The number of positively (“+”, Arg/Lys/N-terminus) and negatively (“−“, Asp/Glu/C-terminus) charged groups in the protein at neutral pH. The number of His residues, which could change their ionization state at a pH of about 6.0, is also shown. b Mean Kyte-Doolittle hydrophobicity index [45]. The maximum and minimum values of this index are +4.5 and −4.5 for poly-Ile and poly-Arg sequences, respectively. c 1H frequency of the NMR spectrometer, where the 15N relaxation was measured. d First value shows the sample, its concentration, or temperature used for structure calculation; the second shows the same value for the 15N relaxation measurements. e 5% dioxane was added to the sample to prevent aggregation.