Table 2.
Rotational diffusion of TFPs in solution.
| Protein | T, °C | Theoretical a | Experimental b | |||
|---|---|---|---|---|---|---|
| τR, ns | Anisotropy 2Dz/(Dx + Dy) | Asymmetry Dx/Dy |
τR, ns (iso/axial) |
Anisotropy 2Dz/(Dx + Dy) | ||
| trans-SLURP-1 | 37 | 5.0 | 1.56 | 1.06 | 4.3/4.9 | 1.50 |
| cis-SLURP-1 | 37 | 5.1 | 1.44 | 1.11 | 4.3/4.8 | 1.78 |
| Lypd6 | 37 | 6.1 | 1.30 | 1.10 | 4.6/4.6 | 1.26 |
| Lypd6b | 37 | 6.1 | 1.36 | 1.11 | 5.0/5.0 | 1.32 |
| Lynx2 | 37 | 3.7 | 1.25 | 1.17 | 4.0/3.8 | 1.35 |
| Lynx1 | 25 | 6.1 | 1.56 | 1.14 | 5.0/5.2 | 1.57 |
| SLURP-2 | 37 | 4.0 | 1.60 | 1.01 | 4.0/4.1 | 1.42 |
| WTX-P33A | 30 | 3.7 | 1.49 | 1.06 | 3.6/3.6 | 1.34 |
| NTII | 30 | 3.7 | 1.50 | 1.08 | 2.9/3.0 | 1.42 |
a The values were calculated using the three-dimensional (3D) structures of proteins and the HYDRONMR program [52]. b The values were calculated in the FastModelFree program [53] using the 15N relaxation data assuming isotropic or axially symmetric rotational diffusion tensor. The values shown for axially symmetric rotational model are the average of three independent calculations using different protein structures.