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. 2020 Oct 6;21(19):7373. doi: 10.3390/ijms21197373

Figure 2.

Figure 2

Neuroprotective effects of LE or vehicle on the MCAO and reperfusion injury after the administration of DMSO or XAV939. (a) TTC-stained brain slices for infarction measurement. Decrease in infarction volume was observed in the DMSO+MCAO+LE 20% group but not in the XAV939+MCAO+LE 20% group. Sham group (DMSO+Sham+Veh) did not experience ischemic reperfusion injury; (b) Measurement of infarction volume by TTC staining. The DMSO+MCAO+Veh group exhibited significant increase in infarction volume compared to the DMSO+Sham+Veh group. DMSO+MCAO+LE 20% group decreased significantly with respect to infarction volume when compared to the DMSO+MCAO+Veh. The XAV939+MCAO+LE 20% group had significantly increased infraction volume compared to the DMSO+MCAO+LE 20% group; (c) Bederson scores of experimental groups. The DMSO+MCAO+Veh group significantly increased in Bederson scores compared to the DMSO+Sham+Veh group. The DMSO+MCAO+LE 20% group showed significant decrease in Bederson scores compared to the DMSO+MCAO+Veh groups. The XAV939+MCAO+LE 20% group significantly increased in Bederscon scores compared to the DMSO+MCAO+LE 20% group. Data are presented as mean ± standard error of the mean (SEM); n = 16 for each group; *** p < 0.001 vs. DMSO+Sham+Veh, # p < 0.05 vs. DMSO+MCAO+Veh, † p < 0.05 vs. DMSO+MCAO+LE 20%. Statistical analysis for the measurement of infarction volume was performed using one-way ANOVA followed by Tukey’s multiple comparison test. Statistical analysis for Bederson scores was performed using Kruskal-Wallis non-parametric test followed by Dunn’s post hoc test.