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. 2020 Oct 6;21(19):7373. doi: 10.3390/ijms21197373

Figure 3.

Figure 3

Effects of LE or vehicle on protein expressions after MCAO and reperfusion injury. (a) Representative Western blots indicating the expression of specific proteins in the penumbra region of the left hemisphere; (b–d) Expression and phosphorylation of Akt in the experimental groups. pAkt levels in MCAO+Veh group decreased significantly compared to the Sham+Veh group. pAkt was significantly increased in the MCAO+LE 20% group compared to the MCAO+Veh group; (e–g) Expression and phosphorylation levels of GSK-3β (pGSK-3β) in the experimental groups. pGSK-3β and GSK-3β levels in the MCAO+Veh group was significantly lower than the Sham+Veh group. The MCAO+LE 20% had significantly increased pGSK-3β and GSK-3β levels compared to the MCAO+Veh group; (h–m) Wnt signal-related protein expressions of experimental groups. Decreased levels of Wnt1, Wnt3, PORCN and β-catenin were observed in the MCAO+Veh group compared to the Sham+Veh group. The MCAO+LE 20% group had significantly increased protein levels of Wnt1, PORCN and β-catenin compared to MCAO+Veh group. Increased levels of pβ-catenin and tankyrase 1 were observed in the MCAO+Veh group compared to the Sham+Veh group. The MCAO+LE 20% group had significantly decreased levels of pβ-catenin and tankyrase 1 compared to the MCAO+Veh group; (n–q) Inflammatory protein expressions of experimental groups. Significantly increased expressions of inflammatory markers were observed in MCAO-injured groups compared to the Sham+Veh group. The MCAO+LE 20% group had significantly decreased inflammatory protein expression levels compared to the MCAO+Veh group. Data are presented as mean ± standard error of the mean (SEM); n = 8 for each group; * p < 0.05, ** p < 0.01 vs. Sham+Veh, # p < 0.05, ### p < 0.001 vs. MCAO+Veh, one-way ANOVA followed by Tukey’s multiple comparison test.