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. 2020 Oct 6;21(19):7373. doi: 10.3390/ijms21197373

Figure 4.

Figure 4

Effects of LE or vehicle on protein expression on the MCAO and reperfusion injury after the administration of DMSO or XAV939. (a) Representative Western blots of proteins in the penumbra region of the left hemisphere; (b–d) Levels of Akt and pAkt in the experimental groups. The DMSO+MCAO+Veh group and XAV939+MCAO+LE 20% group decreased significantly in pAkt level compared to DMSO+Sham+Veh group. pAkt was significantly increased in the DMSO+MCAO+LE 20% group compared to the DMSO+MCAO+Veh group and XAV939+MCAO+LE 20% group; (e–g) Levels of GSK-3β and pGSK-3β in the experimental groups. GSK-3β and pGSK-3β levels of DMSO+MCAO+Veh group and XAV939+MCAO+LE 20% group were significantly lower than the DMSO+Sham+Veh group. The DMSO+MCAO+LE 20% had significantly increased pGSK-3β and GSK-3β levels compared to the DMSO+MCAO+Veh and XAV939+MCAO+LE 20% groups; (h–m) Wnt signal-related protein expressions of experimental groups. Significantly decreased levels of Wnt1, Wnt3, PORCN and β-catenin were observed in the DMSO+MCAO+Veh group compared to the DMSO+Sham+Veh group. The DMSO+MCAO+LE 20% group had significantly increased protein levels of Wnt1 and PORCN compared to the DMSO+MCAO+Veh group. The XAV939+MCAO+LE 20% group had decreased expression levels of Wnt1 and PORCN compared to the DMSO+MCAO+LE 20% group. The β–catenin expression level in the DMSO+MCAO+LE 20% and XAV939+MCAO+LE 20% groups did not differ significantly. There was a significant increase in pβ–catenin and tankyrase 1 in the DMSO+MCAO+Veh compared to the DMSO+Sham+Veh group. Pβ–catenin and tankyrase1 was significantly decreased in the DMSO+MCAO+LE 20% compared to the DMSO+MCAO+Veh group. The XAV939+MCAO+LE 20% group had significantly increased pβ–catenin and tankyrase 1 expression levels compared to the DMSO+MCAO+LE 20% group; (n–q) Inflammatory protein expressions in the experimental groups. Significantly increased expressions of inflammatory markers were observed in the MCAO-injured groups compared to the DMSO+Sham+Veh group. The DMSO+MCAO+LE 20% group had significantly decreased inflammatory protein expression levels compared to the DMSO+MCAO+Veh group. Significant decrease in inflammatory protein expressions were observed in the XAV939+MCAO+LE 20% group compared to the DMSO+Sham+Veh group. TNF-α expression level was increased significantly in the XAV939+MCAO+LE 20% group compared to the DMSO+MCAO+LE 20% group. Data are presented as mean ± standard error of the mean (SEM); n = 8 for each group; * p < 0.05, ** p < 0.01 vs. DMSO+Sham+Veh, # p < 0.05, ### p < 0.001 vs. DMSO+MCAO+Veh, † p < 0.05, ††† p < 0.001 vs. DMSO+MCAO+LE 20%, one-way ANOVA followed by Tukey’s multiple comparison test.