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. 2020 Oct 22;10:18093. doi: 10.1038/s41598-020-75172-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Rapid, multiscale MPM ex vivo imaging of freshly excised facial skin at sub-micron resolution. (a) Photographic image of the excised facial skin. (b) MPM map overview of the skin tissue epidermis generated by strip-mosaic scanning; the 80 MPx image was acquired at 25 μm depth as a single frame over a 1.2 × 1.0 cm² area and restored with CARE network in 2.15 min. (c) Tile mosaic image of the orange outlined area in (a) and (b) covering 4.5 × 4.5 mm² (25 MPx, acquired and restored with CARE in 2.5 min) with hairs outlined by dashed circles. (d) Digital zoom in of the 1 × 1 mm² yellow outlined area in (c) showing a close-up of the skin folds, hairs and keratinocytes within the epidermis. (e) 1 MPx image acquired in 2 s by optically zooming into the 400 × 400 μm² blue outlined area in (d). The image shows well-resolved keratinocytes surrounding a hair follicle. (f) Optical zoom in at higher rate sampling 1 MPx, 200 × 200 μm² image allows visualization of intracellular features and protective melanin rings around the nuclei of keratinocytes (200 × 200 μm², 1 MPx image acquired in 2 s).