Figure 5.

Migration of chemerin isoform-overexpressing Hepa1-6, HepG2 and Huh7 cells. (A) Images of the cells at 72 h (Hepa1-6, HepG2) or 48 h (Huh7) after scratching. Number of migrated (B) Hepa1-6, (C) HepG2 or (D) Huh7 cells expressing human chemerin isoforms at 48 h (Huh7) or 72 h (Hepa1-6, HepG2) after scratching. Data were analyzed with one-way ANOVA with post-hoc Tukey test. C are cells transfected with the insertless plasmid. % migration determined by analysis of wound healing in (E) Hepa1-6 and (F) HepG2 cells at 72 h post-transfection or (G) Huh7 cells at 48 h post-transfection. * p < 0.05; ** p < 0.01; n = 3. The statistical test used was the Welch test with post-hoc Games–Howell test.