Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 1;25(19):4501. doi: 10.3390/molecules25194501

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Proof of feasibility: FRET imaging of O-GlcNAcylated GFP-3a-β-catenin. (a) SLiM-FRET experiment using two-photon excitation: representative photon decay curves and associated fluorescence mean lifetime extracted from only the GFP emission channel. (b) Donor dequenching after acceptor photobleaching. The inserted blue box indicates the photobleached area. Histograms correspond to calculated pb-FRET inside the cells after photobleaching in comparison to Ac₄GalNAl and Ac₄ManNAz, which serve as a negative control.