Fig. 5.
Superoxide production and NADPH consumption by TGR. TGR (500 nM) was incubated with NADPH (100 μM) and compounds 1 and 4 for 30 min. Samples were desalted to remove unreacted compounds. NADPH consumption and superoxide production using pyrogallol red of the inhibited enzymes were determined. Addition of 10 units of SOD abolished oxidation of pyrogallol red (not shown).