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. 2020 Sep 29;21(19):7216. doi: 10.3390/ijms21197216

Figure 4.

Figure 4

DMF and pio differently regulate the antioxidant enzymes heme oxygenase 1 (HO-1) and MnSOD. OP cultures treated for 24 h (unless indicated) with 10-µM DMF or 1-µM pio were immunostained for MnSOD (A). The micrographs show MnSOD (green) and Hoechst 33258 (blue, H). The bar graph shows MFI analysis of the CTR and treated cells (n = 250–300 cells; * p < 0.05 vs. CTR). WB analysis of MnSOD expression is shown (B). Representative bands of MnSOD and β-actin are shown above. Mean and individual data points from the densitometric analysis are shown in the graph below (n = 3; * p < 0.05 vs. CTR). OP cultures were treated with 10-µM DMF or 1-µM pio and then processed for IF experiments for HO-1 (C). The micrographs show HO-1 (green). The bar graph shows MFI (n = 250-300 cells; * p < 0.05 vs. CTR). WB analysis of HO-1 expression is shown (D). Representative bands of β-actin and HO-1 are shown above. Mean and individual data points from the densitometric analysis are shown in the graph below (n = 3; * p < 0.05 vs. CTR).