Table 1.
Substrate preference of recombinant Perilla frutescens O-methyltransferase3 (PfOMT3).
| Substrate | Relative Activity (%) a |
|---|---|
| Flavanone | |
| Naringenin | 97.79 |
| Eriodictyol | 53.13 |
| Taxifolin | ND b |
| Flavone | |
| Apigenin | 95.17 |
| Luteolin | 46.80 |
| Chrysin | 100.00 |
| 4′,7-Dihydroxyflavone | 62.76 |
| Flavonol | |
| 3,6-Dihydroxyflavone | ND b |
| Kaempferol | 40.34 |
| Quercetin | ND b |
a To determine relative activity, 50 µM of each substrate, 100 µM SAM and 50 µg of recombinant PfOMT3 were combined in 20 mM Tris-HCl buffer (pH 7.5). b ND indicates that 7-O-methylated products were not detected in the PfOMT3 reaction mixture.