KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| DENV2-specific IgG 1662 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 1671-1 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 1671-4 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 1615 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 1652 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 1585 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 1591 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 1660 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 1636 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 1606 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 1678 | Nivarthi et al., 2019 | N/A |
| DENV2-specific IgG 2D22 | Gallichotte et al., 2018 | N/A |
| DENV2-specific IgG 3F9 | Gallichotte et al., 2018 | N/A |
| DENV-4G2 (hybridoma-produced Ig) | ATCC HB-112 | Cat# D1-4G2-4-15 |
| DENV-2H2 (hybridoma-produced Ig) | ATCC HB-114 | Cat# D3-2H2-9-21 |
| Anti-Mouse IgG (H+L) Antibody, Human Serum Adsorbed and Peroxidase-Labeled | SeraCare | Cat# 5450-0011 |
| Bacterial and Virus Strains | ||
| DENV2 prM and E: Tonga, 1974, American | This manuscript | GenBank ID: AY744147 |
| DENV2 prM and E: Thailand, 1974, Asian I | This manuscript | GenBank ID: GU289914 |
| DENV2 prM and E: Thailand, 2016 Asian I | This manuscript | GenBank ID: LC410185 |
| DENV2 prM and E: California, 2009, Asian II | This manuscript | GenBank ID: HQ541798 |
| DENV2 prM and E: Puerto Rico, 2003, Asian-American | This manuscript | GenBank ID: EU687235 |
| DENV2 prM and E: India, 2001, Cosmopolitan | This manuscript | GenBank ID: DQ448237 |
| DENV2 prM and E: Sri Lanka, 2004, Cosmopolitan | This manuscript | GenBank ID: GQ252677 |
| DENV2 prM and E: Singapore, 2009, Cosmopolitan | This manuscript | GenBank ID: KM279605 |
| DENV2 prM and E: Sri Lanka, 2016, Cosmopolitan | This manuscript | GenBank ID: KY495803.1 |
| DENV2 prM and E: Guinea, 1981, Sylvatic | This manuscript | GenBank ID: EF105378 |
| DENV2 prM and E: Malaysia, 2008, Sylvatic | This manuscript | GenBank ID: FJ467493 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Fetal Bovine Serum, ultra-low IgG | Thermo Fisher Scientific | Cat#16250078 |
| 100x Penicillin Streptomycin Glutamine | Thermo Fisher Scientific | Cat#10378016 |
| DMEM, high glucose, GlutaMAX Supplement | Thermo Fisher Scientific | Cat#10566-024 |
| Dulbecco’s Phosphate-Buffered Saline, 1X withcalcium and magnesium | Corning Life Sciences | Cat#21–030-CM |
| Dubelcco’s modified Eagle’s/Ham’s F-12 50/50 Mix | GIBCO | 10-092-CV |
| Opti-MEM I | GIBCO | 31985-070 |
| Non-fat dry milk | Bio-Rad | Cat#1706404 |
| Recombinant DNA | ||
| Primer: DENV2_forw_1 5′-GCA GGC ATG ATC ATT ATG TTG ATT C | This manuscript | N/A |
| Primer: DENV2_forw_2 5′-CAC GAA CTG AAA CAT GGA TGT CAT C | This manuscript | N/A |
| Primer: DENV2_forw_3 5′-GCT GCC CAA CAC AAG GAG AAC CTA G | This manuscript | N/A |
| Primer: DENV2_forw_4 5′-GAA AAT AAA GCT TGG CTG GTG CAC A | This manuscript | N/A |
| Primer: DENV2_forw_5 5′-GTT CTC CAT GTA AGA TCC CTT TTG A | This manuscript | N/A |
| Primer: DENV2_forw_6 5′-CAC CAA GTT TTC GGA GCA ATC TAT G | This manuscript | N/A |
| Primer: DENV2_reve_1 5′-CTC AAG ATC CAA GTT TCA ATT CTC T | This manuscript | N/A |
| Primer: DENV2_reve_2 5′-CAG ACG AAC CTT TTG TCC TGC TCT TC | This manuscript | N/A |
| Primer: DENV2_reve_3 5′-CAA TTT GAT CCT TGT GTG TCC GCT C | This manuscript | N/A |
| Primer: DENV2_reve_4 5′-GTA ATC AGG CGA CCT AAA ACA TGT C | This manuscript | N/A |
| Primer: DENV2_reve_5 5′-CTA TCC ATG TGA TAA TGA CTC CTA TG | This manuscript | N/A |
| Primer: DENV2_reve_6 5′-CTC CTG TCA TGA TAG TTA ACT TCA C | This manuscript | N/A |
| Experimental Models: Cell Lines | ||
| Vero | ATCC | CCL-81 |
| C6/36 cells | ATCC | CRL-1660 |
| Critical Commercial Assays | ||
| QIAamp Viral RNA Mini Kit | QIAGEN | Cat# 52904 |
| QIAprep Spin Miniprep Kit | QIAGEN | Cat# 27106 |
| mMESSAGE mMACHINE T7 Transcription Kit | Thermo Fisher Scientific | Cat# AM1344 |
| Deposited Data | ||
| N/A | N/A | N/A |
| Software and Algorithms | ||
| GraphPad Prism 7.2 | GraphPad Software Inc. | https://www.graphpad.com |
| PyMOL | Schrödinger, Inc. | https://www.schrodinger.com |
| Other | ||
| Immunospot | C.T.L. | N/A |