Figure 7.

Effect of high-sucrose diet over the insulin-signaling pathway in peripancreatic and gonadal adipose tissue. Representative blots of IRS1, AKT, p-AKT(S473), P70S6K1, p-P70S6K1(T389), and β-actin as load control, from pooled peripancreatic adipose tissue (pWAT) (A) and gonadal adipose tissue (gWAT) (C) from control and HSD groups. Normalized ratio of phosphorylated AKT and P70S6K1 and total forms in pWAT (B) and gWAT (D). Mean density ± s.e.m. from three independent experiments. Two-way ANOVA was performed for p-AKT (Diet F(1,8) = 22.4, P = 0.0014) *P < 0.01 compared to control; p-P70S6K1 (Diet F(1,8) = 7.89, P = 0.022) with post hoc Tukey’s test with interaction between sex and diet. *P < 0.05 compared to control in pWAT. For p-AKT in gWAT (Sex F(1,8) = 7.51, P = 0.02). One-way ANOVA with post hoc Tukey’s test *P < 0.008 compared to control.