Figure 8.

Effect of high-sucrose diet over the insulin-signaling pathway in gastrocnemius muscle. Representative blots of AKT, p-AKT(S473), P70S6K1, p-P70S6K1(T389) and β-actin as a load control, from pooled gastrocnemius muscles (A). Normalized ratio of phosphorylated AKT(S473) and P70S6K1(T389) and total forms (B). Mean density ± s.e.m. from three independent experiments. Two-way ANOVA was performed for p-AKT (Sex, F(1,4) = 0.309, P = 0.60 and diet F(1,4) = 68.92, P = 0.001), with post hoc Tukey’s test *P < 0.003 compared to control. With interaction between sex and diet.