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. 2020 Oct 23;20:484. doi: 10.1186/s12870-020-02692-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Metabolomics analysis of tree peony buds. a Bud morphology of tree peony buds during the chilling duration. The scale bar indicated 1 cm. b PCA plot of the tree peony buds at five stages. 0 d, 7 d, 14 d, 21 d and 28 d indicated days for chilling duration. QC, quality control samples. c The KEGG pathway annotation of metabolites. Black texts indicated the first hierarchy of KEGG Pathway database, and colored texts indicated the second hierarchy of KEGG Pathway database. The global and overview is a secondary metabolism name, containing eight metabolic pathways: Metabolic pathways, Biosynthesis of secondary metabolites, Microbial metabolism in diverse environments, Carbon metabolism, 2-Oxocarboxylic acid metabolism, Fatty acid metabolism, Biosynthesis of amino acids, Degradation of aromatic compounds