Table 1.
Major putative QTL (R2 > 10.0%) associated with soybean seed protein concentration identified by multiple QTL mapping (MQM) in the two RIL populations (‘AC X790P x S18-R6’ and ‘AC X790P x S23-T5’) evaluated in five environments (CHA15, CHA16, MER15, MER16 and PAL16)
| QTL Namea | Chr. | POPn | Flanking Markers | Size (cM) | LODb | Ac | R2 (%) | Source | Referencesd | |
|---|---|---|---|---|---|---|---|---|---|---|
| qPro_Gm01–2 | 1 | 2 | S01_42371693 | S01_42555910 | 2.19 | 4.56 | 0.4578 | 10.4 | S23-T5 | – |
| qPro_Gm02–3 | 2 | 1 | S02_40793724 | S02_41072417 | 4.58 | 5.16 | 0.4115 | 10.4 | AC X790P | VALSMA; 1,2 |
| qPro_Gm04–3 | 4 | 2 | S04_44592458 | S04_45008840 | 1.64 | 5.25 | 0.4931 | 11.0 | S23-T5 | 2, 3, 11 |
| qPro_Gm04–4 | 4 | 1 | S04_48435528 | S04_49024162 | 14.21 | 6.03 | 0.3570 | 13.7 | AC X790P | – |
| qPro_Gm05–2 | 5 | 1 | S05_38330071 | S05_38993543 | 12.31 | 6.80 | 0.4132 | 14.2 | AC X790P | VALSMA |
| qPro_Gm06–1 | 6 | 1 | S06_19074 | S06_699413 | 1.68 | 10.19 | 0.4408 | 21.9 | S18-R6 | – |
| qPro_Gm06–3 | 6 | 1 | S06_9128442 | S06_11029737 | 19.08 | 5.51 | 0.3339 | 12.6 | S18-R6 | VALSMA |
| qPro_Gm06–6 | 6 | 1 | S06_30639643 | S06_33589987 | 0.28 | 5.80 | 0.3046 | 13.2 | AC X790P | 2, 5, 6, 7 |
| qPro_Gm08–2 | 8 | 1 | S08_43864875 | S08_43896183 | 2.25 | 5.38 | 0.3936 | 12.3 | AC X790P | VALSMA |
| qPro_Gm12–3 | 12 | 1 | S12_924424 | S12_1147989 | 11.46 | 6.45 | 0.4943 | 11.6 | S18-R6 | – |
| qPro_Gm12–4 | 12 | 1 | S12_3518939 | S12_3666689 | 7.64 | 6.63 | 0.4757 | 12.0 | S18-R6 | – |
| qPro_Gm13–4 | 13 | 2 | S13_28227783 | S13_28254683 | 4.46 | 8.54 | 2.2804 | 11.6 | AC X790P | VALSMA |
| qPro_Gm15–3 | 15 | 2 | S15_10218629 | S15_10877491 | 1.64 | 5.63 | 0.6925 | 11.5 | AC X790P | VALSMA; 4,8,9,10 |
| qPro_Gm18–4 | 18 | 1 | S18_52660341 | S18_53019901 | 18.54 | 4.50 | 0.2713 | 10.4 | AC X790P | VALSMA; 2 |
aQTL for the same trait detected in all individual environments (CHA15, CHA16, MER15, MER16 and PAL16) and the combined environment (GMET) with the same or overlapping marker interval was designated as one QTL. QTL highlighted in bold are novel QTL and were validated in the other RIL population
bLOD thresholds were calculated through a permutation test with 1000 iterations and a Type I error rate of 0.001
cAdditive effects calculated as the absolute value of half the subtraction of the mean of genotypes with the ‘S18-R6’ (‘POPn_1’) or ‘S23-T5’ (POPn_2) allele (negative effect) from the mean of genotypes with the ‘AC X790P’ allele (positive allele)
dIndicating that the QTL was confirmed in the other RIL population through multiple QTL mapping (VALMQM), single marker analysis (VALSMA), and/or has been reported previously in the reference(s): 1. [31] 2. [30] 3. [32] 4. [28] 5. [34] 6. [36] 7. [35] 8. [26] 9. [29] 10. [27].11. [33]