FIG. 6.

TERT monoallelic expression in heterozygous TERT mutant thyroid cancer cell lines. Sanger sequencing analysis of the exon 2 region and 3′ UTR region of TERT surrounding SNPs rs2736098 and rs2853690, respectively, in amplified genomic DNA, as well as cDNA, from TERT heterozygous mutant cell lines TPC-1, BCPAP, and FTC-238. While the genomic DNA in all cell lines showed both alleles of the SNP, the cDNA showed only one allele in all cell lines. cDNA, complementary DNA; SNP, single nucleotide polymorphism; UTR, untranslated region.