Table 2.
Specification and overview of the experimental setups used for visualization of biofilm formation in the human plasma biofilm model (hpBIOM) using scanning electron microscopy (SEM).
| Image | Setup/substance | Pathogen | Maturation time* | Treatment period |
|---|---|---|---|---|
| 4 (a) | CTRL | P. aeruginosa | 12 h | - |
| 4 (b) | CTRL | P. aeruginosa | 18 h | - |
| 4 (c) | CTRL | P. aeruginosa | 36 h | - |
| 5 (a) | CTRL | P. aeruginosa | 12 h | 0 h |
| 5 (b) | OCT/PE | P. aeruginosa | 12 h | 6 h |
| 5 (c) | OCT/PE | P. aeruginosa | 12 h | 24 h |
| 5 (d) | NaOCl (<0.08%) | P. aeruginosa | 12 h | 24 h |
Figures 4A–C demonstrate a representative biofilm development over time. Figures 5A–D show the biofilm surface structure under treatment with antiseptics (OCT/PE) or antimicrobial wound irrigation solution (<0.08% NaOCl) over time compared to the initial structure.
*
Before application of test substance, if any (not in case of CTRL).