Figure 3.

Effect of intra-lymphatic injection of GAD-alum (n = 12) on T cells and cells with regulatory phenotype. (A) Median percentage of activated (CD25⁺CD127⁺) CD4⁺ (blue triangles) and CD8⁺ (orange circles) T cells after stimulation with GAD₆₅ (5 µg/ml). (B) Gating strategy for naïve (T_N, CD45RA⁺CCR7⁺, gray circles), central memory (T_CM, CD45RA^-CCR7⁺, orange squares), and effector memory (T_EM, CD45RA^-CCR7^- and CD45RA⁺CCR7^-, blue triangles) cells, and median percentage within CD4⁺ (up) and CD8⁺ (down) T cells. (C) Representative dot plot showing the expression of FOXP3 and CD25 on CD4⁺ T cells and median percentage of regulatory T cells within CD4⁺ T cells. (D) Gating strategy and median percentages for non-suppressive (nsTreg, CD45RA^-FOXP3^low, blue circles), resting (rTreg, CD45RA⁺FOXP3^low, gray squares), and activated (aTreg, CD45RA^-FOXP3^high, orange triangles) regulatory T cells. Bars indicate interquartile range. Differences between time-points within the same group were calculated using Wilcoxon paired test.