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Serial dilution series of lentivirus stock. Prepare a 0.1 dilution by transferring 2 μl of the undiluted virus stock into a single 1.5‐ml Eppendorf tube containing 18 μl PBS. Mix well. Using a new pipet tip, transfer 2 μl of this dilution into a new 1.5‐ml Eppendorf tube containing 18 μl PBS to prepare the 0.01 dilution.
