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. 2020 Sep 28;133(1):e125. doi: 10.1002/cpmb.125

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© 2020 The Authors.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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qPCR analysis for lentivirus titration. Representative triplicate Cq values for the LV2 and albumin genes are averaged to calculate the Cq mean for a serial dilution of lentivirus of known (A) or unknown (B) titration. A delta (Δ) Cq is calculated by subtracting the LV2 Cq mean from the albumin Cq mean. The Cq mean values for the lentivirus of known (C) and unknown (D) titration are plotted on an XY graph against dilution value. A natural log regression analysis is performed, and the unknown variables are used to calculate the unknown lentivirus titer. The letters (T‐W, Z) denote variables to be solved using the formula.