Extended Data Fig.11|. Gating strategies, summary and potential applications of senolytic CAR T cells.

(a)-(b) Representative flow cytometric staining of m.uPAR-h.28z CAR T cells (a) or untransduced T cells (b) obtained from the livers of mice that had undergone hydrodynamic tail vein injections (HTVI) (as depicted in Fig.2). Representative results of 1 independet experiment (n=4 mice per group). (c) Illustration summarizing key points of our findings. uPAR-28z CAR T cells (depicted in red) infiltrate fibrotic livers containing senescent cells (here depicted in blue) and efficiently eliminate them, leading to fibrosis resolution and improved liver function. The therapeutic action of senolytic uPAR-28z CAR T cells might be extended to other senescence-associated diseases such as lung fibrosis, atherosclerosis, diabetes or osteoarthritis. Images were created with Biorender.com.