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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Nature. 2020 Jun 17;583(7814):127–132. doi: 10.1038/s41586-020-2403-9

Figure 4|. Senolytic CAR T cells are therapeutic in NASH-induced liver fibrosis.

Figure 4|

(a,b) Representative stainings in the livers of mice treated with chow or NASH diet for 3–4 months. (a) Immunohistochemical staining of m.uPAR and SA-β-Gal (n=3 mice per group) (b) Co-immunofluorescence staining of m.uPAR (green), desmin (red in left panel, gray in right panel) and F4/80 (red). Representative results of n=2 independent experiments (n=3 mice per group). (c-d) Mice treated with NASH diet for 3 months were injected with 0.5×106 m.uPAR-m.28z CAR T cells or untransduced (UT) T cells. Liver and serum analyses were performed 20 days later. (c) Representative Sirius red staining, SA-β-Gal expression and quantifications (Sirius red: UT: n=9 mice, m.uPAR-m.28z: n=11. SA-β-Gal: UT: n= 4 mice, m.uPAR-m.28z: n=6). (d) Serum albumin levels (UT and m.uPAR-m.28z: n=6). (c-d) Results of n=2 independent experiments. Data are mean± SEM. Two-tailed unpaired Student’s t-test.