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. 2020 Oct 23;89:107408. doi: 10.1016/j.compbiolchem.2020.107408

Fig. 6.

Fig. 6

Concentration-dependent inhibition of the SARS-CoV-2 Mpro by hypericin and cyanidin-3-O-glucoside. To confirm in vitro inhibition a 3CL protease ELISA assay was performed and fluorescence intensities at an emission of wavelength of 460 nm for concentrations of hypericin and cyanidin-3-O-glucoside up to 128 μM (doubling dilutions), were measured. The average background (n = 9 determinations), total enzymatic activity (n = 9 determinations), and inhibition by the covalent internal positive control, GC376 at 50 μM (n = 9 determinations), are highlighted (horizontal dashed lines). Hypericin (closed circles) and cyanidin-3-O-glucoside (open circles) were assayed in triplicate and the average ± SEM values are depicted. Concentration-dependent inhibition of the SARS-CoV-2 Mpro by hypericin and cyanidin-3-O-glucoside. To confirm in vitro inhibition a 3CL protease ELISA assay was performed and fluorescence intensities at an emission of wavelength of 460 nm for concentrations of hypericin and cyanidin-3-O-glucoside up to 128 μM (doubling dilutions), were measured. The average background (n = 9 determinations), total enzymatic activity (n = 9 determinations), and inhibition by the covalent internal positive control, GC376 at 50 μM (n = 9 determinations), are highlighted (horizontal dashed lines). Hypericin and cyanidin-3-O-glucoside were assayed in triplicate and the average ± SEM values are depicted.