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. 2020 Jun 22;31(8):1928–1937. doi: 10.1021/acs.bioconjchem.0c00284

Figure 3.

Figure 3

Knockdown of c-FLIP by antisense PNA-Arg8 conjugates (PNA1–5-Arg8) targeted against different mRNA positions. (A) Placement of anti-c-FLIP-PNAs relative to the AUG start codon. (B) Western blot of lysate from A549 cells treated with 2.5 μM PNA conjugates or OptiMEM (C) for 6 h followed by incubation in medium for 18 h. Protein samples were resolved on SDS-PAGE and Western immunoblotted with antibodies against short (C-FLIPS) and long (c-FLIPL) isoforms of c-FLIP and a β-actin loading control.