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. Author manuscript; available in PMC: 2020 Oct 23.
Published in final edited form as: Arch Pathol Lab Med. 2016 Sep 2;140(11):1200–1205. doi: 10.5858/arpa.2016-0108-RA

Figure 2.

Figure 2.

Repurposing of the unused cytology material for molecular testing. Key steps in the process are illustrated. A, Cytology suspension in CytoLyt (Cytyc Corporation, Marlborough, Massachusetts) is pelleted by gentle centrifugation (600g) to preserve cell morphology. B through D, The pellet is processed into a cell block using a modified HistoGel (Thermo Scientific, Waltham, Massachusetts) method. E, An aliquot of the pellet is transferred into a PreservCyt container (Hologic, Bedford, Massachusetts) and used to prepare a ThinPrep slide (Hologic). F through H, The CytoLyt supernatant and residual PreservCyt are combined. Isopropanol is added to precipitate cell-free DNA, followed by high-speed (17 000–18 000g) centrifugation. The pelleted material is transferred into a microcentrifuge tube for extraction and storage.