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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Methods Mol Biol. 2021;2219:195–216. doi: 10.1007/978-1-0716-0974-3_12

Figure 3. Examples of Combination of immunolabeling and in situ hybridization in two sea urchin species.

Figure 3.

FISH and IHC can be used to study RNA and protein localization for the same gene of interest. A-A”) show that the RNA for the neuropeptide ANP2 is everywhere in the cell, while the ANP2 protein is enriched at the cell apical side (see magnification in white boxes in C”). B-B”) show stable microtubule staining paired with a FISH for the stomach gene ManRC1A. C-C”) show immunolabeling of the neuropeptide Trh and orthopedia (Otp). Dashed boxes indicate the region magnified in the white box. Scalebars are 20 μm for sea urchins, 50 μm for sea star, and 5μM for the magnifications in the white boxes.