KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| TCR V beta 13.1 monoclonal antibody, FITC | Thermo Fisher Scientific | Cat#11–5792-41; RRID: AB_1944415 |
| PE anti-human HLA-A2 antibody | BD Biosciences | Cat#558570; RRID: AB_647220 |
| IFN gamma monoclonal antibody | Thermo Fisher Scientific | Cat#M700A; RRID: AB_223578 |
| IFN gamma monoclonal antibody, Biotin | Thermo Fisher Scientific | Cat#M701B; RRID: AB_223580 |
| Rabbit anti-human IFN gamma antibody | Abcam | Cat#ab9657; RRID: AB_2123314 |
| Human IL-2 biotinylated antibody | R&D Systems | Cat#BAF202; RRID: AB_356218 |
| Recombinant Rabbit anti-human IL-2 antibody | Abcam | Cat#ab92381; RRID: AB_10564139 |
| CD3 monoclonal antibody | Thermo Fisher Scientific | Cat#14–0037-82; RRID: AB_467057 |
| Biological Samples | ||
| Fetal bovine serum | Thermo Fisher Scientific | Cat#26140079 |
| Human AB serum | Millipore Sigma | Cat#H3667 |
| TILs clone (2B2) from patients with melanoma | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| NY-ESO-1157–165 (SLLMWITQC), synthetic | GenScript | N/A |
| NY-ESO-V157–165 (SLLMWITQV), synthetic | GenScript | N/A |
| Recombinant human IFN-γ Protein | Thermo Fisher Scientific | Cat#PHC4031 |
| Recombinant human Interleukin-2 | BIO-RAD | Cat#PHP042 |
| DMEM medium | GE Healthcare | Cat#SH30022.01 |
| RPMI 1640 medium | GE Healthcare | Cat#SH30027.01 |
| AIM V medium | Thermo Fisher Scientific | Cat#12055083 |
| Penicillin-Streptomycin | Thermo Fisher Scientific | Cat#15-140-122 |
| GlutaMAX | Thermo Fisher Scientific | Cat#35050061 |
| Dynabead untouched human CD8 T cells | Thermo Fisher Scientific | Cat#11348D |
| Vybrant cell-labeling Kit (DiO, DiL, DiD) | Thermo Fisher Scientific | Cat#V22889 |
| Calcein, AM | Thermo Fisher Scientific | Cat#C3100MP |
| CellTrace Calcein Red-Orange, AM | Thermo Fisher Scientific | Cat#C34851 |
| Critical Commercial Assays | ||
| Alexa Fluor 488 Tyramide SuperBoost Kit | Thermo Fisher Scientific | Cat#B40943 |
| Alexa Fluor 488 Protein Labeling Kit | Thermo Fisher Scientific | Cat#A20181 |
| Experimental Models: Cell Lines | ||
| Human: HeLa cells | ATCC | CCL-2 |
| Human: Raji | ATCC | CCL-86 |
| Software and Algorithms | ||
| Prism 8.0 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| COMSOL Multiphysics 5.4 | COMSOL | https://cn.comsol.com/comsol-multiphysics/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Microsoft Excel | Microsoft | Microsoft Office Suite 2016 |