Antibodies |
|
|
TCR V beta 13.1 monoclonal antibody, FITC |
Thermo Fisher Scientific |
Cat#11–5792-41; RRID: AB_1944415 |
PE anti-human HLA-A2 antibody |
BD Biosciences |
Cat#558570; RRID: AB_647220 |
IFN gamma monoclonal antibody |
Thermo Fisher Scientific |
Cat#M700A; RRID: AB_223578 |
IFN gamma monoclonal antibody, Biotin |
Thermo Fisher Scientific |
Cat#M701B; RRID: AB_223580 |
Rabbit anti-human IFN gamma antibody |
Abcam |
Cat#ab9657; RRID: AB_2123314 |
Human IL-2 biotinylated antibody |
R&D Systems |
Cat#BAF202; RRID: AB_356218 |
Recombinant Rabbit anti-human IL-2 antibody |
Abcam |
Cat#ab92381; RRID: AB_10564139 |
CD3 monoclonal antibody |
Thermo Fisher Scientific |
Cat#14–0037-82; RRID: AB_467057 |
Biological Samples |
Fetal bovine serum |
Thermo Fisher Scientific |
Cat#26140079 |
Human AB serum |
Millipore Sigma |
Cat#H3667 |
TILs clone (2B2) from patients with melanoma |
This paper |
N/A |
Chemicals, Peptides, and Recombinant Proteins |
NY-ESO-1157–165 (SLLMWITQC), synthetic |
GenScript |
N/A |
NY-ESO-V157–165 (SLLMWITQV), synthetic |
GenScript |
N/A |
Recombinant human IFN-γ Protein |
Thermo Fisher Scientific |
Cat#PHC4031 |
Recombinant human Interleukin-2 |
BIO-RAD |
Cat#PHP042 |
DMEM medium |
GE Healthcare |
Cat#SH30022.01 |
RPMI 1640 medium |
GE Healthcare |
Cat#SH30027.01 |
AIM V medium |
Thermo Fisher Scientific |
Cat#12055083 |
Penicillin-Streptomycin |
Thermo Fisher Scientific |
Cat#15-140-122 |
GlutaMAX |
Thermo Fisher Scientific |
Cat#35050061 |
Dynabead untouched human CD8 T cells |
Thermo Fisher Scientific |
Cat#11348D |
Vybrant cell-labeling Kit (DiO, DiL, DiD) |
Thermo Fisher Scientific |
Cat#V22889 |
Calcein, AM |
Thermo Fisher Scientific |
Cat#C3100MP |
CellTrace Calcein Red-Orange, AM |
Thermo Fisher Scientific |
Cat#C34851
|
Critical Commercial Assays |
Alexa Fluor 488 Tyramide SuperBoost Kit |
Thermo Fisher Scientific |
Cat#B40943
|
Alexa Fluor 488 Protein Labeling Kit |
Thermo Fisher Scientific |
Cat#A20181 |
Experimental Models: Cell Lines |
Human: HeLa cells |
ATCC |
CCL-2 |
Human: Raji |
ATCC |
CCL-86 |
Software and Algorithms |
Prism 8.0 |
GraphPad Software |
https://www.graphpad.com/scientific-software/prism/ |
COMSOL Multiphysics 5.4 |
COMSOL |
https://cn.comsol.com/comsol-multiphysics/ |
ImageJ |
NIH |
https://imagej.nih.gov/ij/ |
Microsoft Excel |
Microsoft |
Microsoft Office Suite 2016 |