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CCK8 test. PDAC cells (panc-1, miapaca-2, aspc-1 cells, 4 × 106) were cocultured with neutrophils (1 × 109) after 12 h of seeding (equal PDAC cells seeded first, neutrophils and lymphocytes were added after 12 h; therefore, the OD value showed a difference at 12 h). Compared with lymphocytes (1 × 109) and the monoculture group, neutrophils show a lethal effect on PDAC cells
