Figure 5.

Expression of E-cadherin in three PDAC cells (Panc-1, MiaPaCa-2 and AsPC-1) was significantly reduced when direct cocultured (Di) or indirect cocultured (In) with neutrophils compared with that monocultured (Mo), while vimentin was significantly increased. The expression of E-cadherin and vimentin in Panc-1 at A and B, MiaPaCa-2 at C and D for AsPC-1 at E and F. To make each group comparable, the fluorescence brightness of the nucleus was set at the same level in the laser confocal imaging of the three groups. Vimentin (expressed in the cell membrane) was highly expressed in the indirect culture group in MiaPaCa-2, resulting in a higher brightness, so that it could not be distinguished in the image whether the expression site was the cell membrane or the nucleus. (＊ P < .05; ＊＊ P < .01; ＊＊＊ P < .001)