Figure 6.

Detection of cytokines in three groups of cell lines (P: Panc-1, M:Miapaca-2, A:Aspc-1) in culture medium. (D: directly cocultured with neutrophils) and (In: indirectly cocultured with neutrophils). Detection of the concentrations of TNF-alpha, IL-6, TGF-beta, IL-12, GM-CSF, MMP, IL-8, EGF and G-CSF in cell culture medium (Fig A, B, C, D, E, F, G, H, I and J, respectively). Among all the cytokines, only TNF-α and TGF-β concurred with the experimental hypothesis: secreted by neutrophils, especially when stimulated by pancreatic cancer cells (1. the secretion of the cytokines was significantly increased in the coculture system; 2. their secretion was positively proportional to the concentration of neutrophils). (＊ P < .05; ＊＊ P < .01; ＊＊＊P < .001)