Figure 5.

Distribution of transition kinetics and effect of CT-tagged EGFP and CT-interacting Gap19 peptide. Mono- and biexponential fits are displayed in blue and red, respectively. (A) In HeLa-Cx43 cells, tc_trans was distributed along a monoexponentially decreasing function characterized by a τ of 5.2 msec (N = 4; N = 15; n = 132). (B) For HeLa-Cx43-EGFP, τ was more than twice as large compared to HeLa-Cx43 (N = 3; N = 6; n = 39). (C) HeLa-Cx43 treated with Gap19 showed a second longer τ compared to the control (N = 3; N = 8; n = 50). (D) Selective analysis of tc_trans for transitions in the subconductance and main state demonstrated that Gap19 significantly slowed down transitions to the subconductance state while leaving main state transitions unaffected (Mann–Whitney U test, #### p ≤ 0.0001). Number of events: HeLa-Cx43 80 pS (129 events), HeLa-Cx43 217 pS (1656 events), HeLa-Cx43 + Gap19 80 pS (193 events), HeLa-Cx43 + Gap19 217 pS (453 events).