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. 2020 Sep 28;21(19):7145. doi: 10.3390/ijms21197145

Figure 7.

Figure 7

17β-estradiol potentiates A2A-mediated cell proliferation via estrogen receptors in female mPEC. (A) Q-PCR for measuring mRNA of estrogen receptor (ER) alpha and beta. Both genes were normalized to mlp37. p = 0.34 between alpha and beta. (B) Representative Western blot and respective densitometry of ERα (~70 kDa), ERβ (~60 kDa), and β-actin (~43 kDa). ERs/β-actin ratio is presented. P = 0.86 between alpha and beta. (C) Cells incubated with 17β-estradiol + CGS-21680 with or without the antagonists MMP (10−6 M × 24 h) or PHTPP (10−6 M × 24 h) for ERα and ERβ, respectively. Dotted line in C represents basal values (i.e., control without stimuli). * p < 0.05 versus control. Values are mean ± SEM, n = 3–6 per group. All experiments were performed in duplicates.